Streptozotocin is an antibiotic that is produced by Stretomyces achromogenes. It is used as an antineoplastic agent and to induce diabetes in experimental animals.

Streptozotocin通过低亲和力的GLUT 2葡萄糖转运体在胰岛β细胞中选择性积累.注射4个月60 mg/kg Streptozotocin会诱导β细胞快速脱粒而不坏死,发生白内障并且在肾的近曲小管中积累糖原.在患有' Streptozotocin 糖尿病'大鼠体内,100 mg/kg Streptozotocin损伤胰腺外分泌细胞,使小的可能分泌的颗粒在β细胞的高尔基体中持续存在.Streptozotocin在大鼠、小鼠和仓鼠体内被发现是致癌的.

Streptozotocin对胰岛β细胞具有毒性。Streptozotocin直接甲基化DNA并具有高度遗传毒性,产生DNA链断裂,碱不稳定位点,非计划DNA合成,DNA加合物,染色体畸变,微核,姊妹染色单体交换和细胞死亡。暴露于15 mM Streptozotocin1小时,然后24小时恢复期诱导鼠胰腺β细胞系INS-1的细胞凋亡。30 mM Streptozotocin引起22%的细胞坏死及17%的细胞凋亡。

Streptozocin (STZ) stock solutions of 50 mg/mL in distilled Water (dWater) are freshly prepared for each experiment[2]. Human and murine cell lines are cultured in triplicate in 96-well plates at a density of 2×104 cells/well in the absence (untreated control) or presence of various concentrations of ALX (20-3000 μg/mL) or STZ (1-3000 μg/mL) for 48 h at 37°C under a humidified atmosphere containing 5% CO2. Cells incubated in complete media including dWater in a final concentration of 0.1% served as control for solvent toxicity and cells incubated in complete medium are used as a control for the experiments. The effects of the tested drugs on tumor cell growth or viability are determined employing the MTT assay in accordance with the manufacturer's instructions. The IC50values (drug concentration that induces 50% inhibition of the cell growth) are calculated using the GraphPad Prism 4 program[2].

18883-66-4

C8H15N3O7

265.222

链脲菌素;U 9889;链脲佐菌素;NSC-85998;STZ;Streptozotocin;Streptozocin

[1]Acer S, et al. Oxidative stress of crystalline lens in rat menopausal model. Arq Bras Oftalmol. 2016 Jul-Aug;79(4):222-5. [2]Bolzán AD, et al. Mutat Res. 2002, 512(2-3), 121-13 [3]Saini KS, et al. Biochem Mol Biol Int, 1996, 39(6), 1229-1236. [4]Iwase M, et al. Tohoku J Exp Med, 1989, 159(2), 83-90. [5]Arison RN, et al. Diabetes, 1967, 16(1), 51-56.

H2O:113.3 mg/mL (427.19 mM),Need ultrasonic and warming
DMSO:30 mg/mL (113.11 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years