产品展厅
产品展厅
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IF, IHC, FC | M | Ascites | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MABD168 |
| Description | Anti-WNT1 Antibody, clone 10C8 |
| Alternate Names |
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| Background Information | Wnt-1, also known as Proto-oncogene Wnt-1 or Proto-oncogene Int-1 homolog, was first identified as a proto-oncogene activated by viral insertion in mouse mammary tumors. The Wnt-1 gene encodes a member of a large family of secreted proteins that are cysteine-rich, glycosylated, and poorly soluble. Wnt-1 serves as a ligand for the RYK coreceptor, resulting in Wnt signaling. The Wnt signalling pathway mediates the levels of cytosolic beta-catenin, a multi-functional protein that associates with membrane-bound E-cadherin as well as several DNA-binding proteins, such as members of the TCF/LEF family. Additionally, it serves a ligand for members of the frizzled family of seven transmembrane receptors and plays a role in development of the central nervous system. |
| Product Information | |
|---|---|
| Format | Ascites |
| Control |
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| Presentation | Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Wnt-1 using this mouse monoclonal antibody, Anti-WNT1 Antibody, clone 10C8 validated for use in western blotting, Immunofluorescence, IHC & Flow Cytometry. |
| Key Applications |
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| Application Notes |
Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in HeLa and 3T3-L1 cells. Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WNT1 in human adrenal tissue. Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected WNT1 in HeLa cells. Optimal working dilutions must be determined by end user. |
| Biological Information | |
|---|---|
| Immunogen | Purified recombinant fragment of human WNT1 expressed in E. Coli. |
| Clone | 10C8 |
| Host | Mouse |
| Isotype | IgG1 |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Unpurified |
| UniProt Number |
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| Molecular Weight | ~41 kDa observed. Uncharacterized bands may appear in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance |
Evaluated by Western Blotting in NIH/3T3, 3T3L1, and HeLa cell lysates. Western Blot Analysis: A 1:500-2,000 dilution of this antibody detected WNT1 in NIH/3T3, 3T3L1, and HeLa cell lysates. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions |
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. |
| Packaging Information | |
|---|---|
| Material Size | 100 μL |
