| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | FC, IP, WB | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4385 |
| Brand Family |
Chemicon® |
| Trade Name |
|
| Description | Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85 |
| Background Information | The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB. |
| Key Applications |
|
| Application Notes |
FACS Analysis: a starting range of 5-20 μg/ml is suggested. Immunoprecipitation Immunoblotting Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | 2102Ep human Embryonal Carcinoma Cells |
| Epitope | blood group antigen Ok(a) |
| Clone | TRA-1-85 |
| Host | Mouse |
| Specificity |
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed. SPECIES REACTIVITIES: Refer to comments regarding specificity |
| Isotype | IgG1 |
| Species Reactivity |
|
| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
|
| Stem Cell Type |
|
| Molecular Weight | 35-69 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |