Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | FC, IP, WB | M | Purified | Monoclonal Antibody |
Description | |
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Catalogue Number | MAB4385 |
Brand Family |
Chemicon® |
Trade Name |
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Description | Anti-TRA-1-85 Antibody, blood group Antigen Ok(a), clone TRA-1-85 |
Background Information | The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells. |
Product Information | |
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Format | Purified |
Control |
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Presentation | Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide |
Quality Level | MQ100 |
Applications | |
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Application | Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB. |
Key Applications |
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Application Notes |
FACS Analysis: a starting range of 5-20 μg/ml is suggested. Immunoprecipitation Immunoblotting Optimal working dilutions must be determined by the end user. |
Biological Information | |
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Immunogen | 2102Ep human Embryonal Carcinoma Cells |
Epitope | blood group antigen Ok(a) |
Clone | TRA-1-85 |
Host | Mouse |
Specificity |
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed. SPECIES REACTIVITIES: Refer to comments regarding specificity |
Isotype | IgG1 |
Species Reactivity |
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Antibody Type | Monoclonal Antibody |
Gene Symbol |
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Stem Cell Type |
|
Molecular Weight | 35-69 kDa |
Product Usage Statements | |
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Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months |
Packaging Information | |
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Material Size | 100 μg |