产品展厅
产品展厅
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | WB, FC, IF, IP, ICC | M | Purified | Monoclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | MAB4381 |
| Brand Family |
Chemicon® |
| Trade Name |
|
| Description | Anti-TRA-1-81 Antibody, clone TRA-1-81 |
| Background Information | Human embryonal carcinoma (EC) cells are the stem cells of teratocarcinomas, and they are key components of germ cell tumors (GCTs). Embryonal carcinoma, as do all human pluripotent stem cells, expresses TRA-1-60 and TRA-1-81 antigens, and although their molecular identities are unknown, they are commonly used as markers of undifferentiated pluripotent human stem cells. |
| Product Information | |
|---|---|
| Format | Purified |
| Control |
|
| Presentation | Liquid in 0.05 M Potassium Phosphate, 0.3 M NaCl, pH 8.0 with 0.05% Sodium Azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-TRA-1-81 Antibody, clone TRA-1-81 detects level of TRA-1-81 & has been published & validated for use in WB, FC, IF, IP & IC. |
| Key Applications |
|
| Application Notes |
Immunofluorescence: A previous lot of this antibody was used in IF. Western Blot: A previous lot of this antibody was used in WB. Immunoprecipitation: A previous lot of this antibody was used in IP. Immunohistochemistry: A previous lot of this antibody was used in IH. Flow Cytometry: A starting range of 10-20 μg/mL is suggested. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Human embryonal carcinoma cell line 2102Ep |
| Clone | clone TRA-1-81 |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Mouse |
| Specificity | This antibody reacts with TRA-1-81 antigen that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. Both the TRA-1-81 and TRA-1-60 monoclonal antibodies (MAB4360) recognize antigens that are associated with a pericellular matrix proteoglycan. TRA-1-60 reacts with a sialidase-sensitive epitope whilst TRA-1-81 reacts with an unknown epitope of the same molecule. |
| Isotype | IgM |
| Species Reactivity |
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| Antibody Type | Monoclonal Antibody |
| Gene Symbol |
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| Purification Method | Ammonium Sulfate Precipitation |
| Stem Cell Type |
|
| Molecular Weight | 420/250 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 μg |
