Species Reactivity | Key Applications | Host | Format | Antibody Type |
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H | ICC, IHC | M | Biotin | Monoclonal Antibody |
Description | |
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Catalogue Number | MAB1273B |
Description | Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate |
Alternate Names |
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Background Information | This antibody recognizes a 60 kDa nonglycosylated protein component of mitochondria found in human cells. It can be used to stain mitochondria in cell, tissue or biochemical preparations and can be used as a marker of the mitochondria in subcellular fractions. MAB1273B recognizes a 65 kD protein by immunoprecipitation and produces the stringy, "spaghetti-like", staining patten in the cytoplasm of human cells. |
Product Information | |
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Format | Biotin |
Control |
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Presentation | Purified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA. |
Quality Level | MQ100 |
Applications | |
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Application | Detect Mitochondrial Marker using this mouse monoclonal antibody, Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate validated for use in ICC & IHC. |
Key Applications |
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Application Notes |
Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs). Immunocytochemsitry Analysis: A 1:50 dilution of this antibody did not detect mitrochondria in primary mouse embryonic fibroblasts (PMEFs). Evaluated by Immunohistochemistry in human kidney tissue. Immunocytochemsitry Analysis: A 1:500 dilution of this antibody detected mitrochondria in human kidney tissue. |
Biological Information | |
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Immunogen | Surface of intact human mitochondria |
Clone | 113-1 |
Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
Host | Mouse |
Specificity | This antibody recognizes human. Antibody is not reactive with mouse or rodent. |
Isotype | IgG1 |
Species Reactivity |
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Species Reactivity Note | Demonstrated to react with human mitochondria protein. Antibody is not reactive with rat or mouse mitochondria protein. |
Antibody Type | Monoclonal Antibody |
Purification Method | Protein G Purified |
Molecular Weight | 60 kDa calculated |
Product Usage Statements | |
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Quality Assurance |
Evaluated by Immunocytochemistry in human adipose mesenchymal stem cells (SCC038). Immunocytochemsitry Analysis: A 1:50 dilution of this antibody detected mitrochondria in human adipose mesenchymal stem cells (SCC038). |
Usage Statement |
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Storage and Shipping Information | |
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Storage Conditions | Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt. |
Packaging Information | |
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Material Size | 50 μL |