ESG1106 Sigma-Aldrich ESGRO® Recombinant Mouse LIF Protein-南京沃博生物科技有限公司

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ESG1106 Sigma-Aldrich ESGRO® Recombinant Mouse LIF Protein

2023-05-05

产品详情

产品名称ESG1106 Sigma-Aldrich ESGRO® Recombinant Mouse LIF Protein 查看相似产品 >

简介

Key Spec Table

Key Applications	Purity
Cult	The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.

Description
Catalogue Number	ESG1106
Brand Family	Chemicon®
Trade Name	Chemicon
Description	ESGRO® Recombinant Mouse LIF Protein
Overview	The ESGRO mouse LIF medium supplement is supplied in active units/mL for consistent performance from lot to lot. Each lot of ESGRO supplement undergoes stringent quality control testing to ensure reliable inhibition of mouse pluripotent stem cell differentiation. ESGRO mouse LIF supplement may be used for feeder based and feeder free culture of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells.
Alternate Names	Murine LIF Mouse LIF mLIF
Background Information	Mouse Leukemia Inhibitory Factor (LIF, mLIF) promotes self-renewal and long-term maintenance of pluripotent mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells by suppressing spontaneous differentiation.

Product Information
Format	Purified
Presentation	ESGRO® Supplement is supplied in liquid form as 106 Units in 1.0 mL of phosphate buffered saline with 1% w/v bovine serum albumin BSA as a carrier for stability.
Quality Level	MQ100

Applications
Application	ESGRO Leukemia Inhibitory Factor (LIF) supplement for mouse ES cell culture. Each vial contains 10^6 units/ml
Key Applications	Stem Cell Culture
Application Notes	Applications for ESGRO® Supplement include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells. SUGGESTED PROTOCOLS: ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® Supplement per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells (E3). At the recommended concentration 107 units of ESGRO® Supplement is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® Supplement is sufficient for 1.0 L of tissue culture media.

Biological Information
Concentration	1 million units/1 mL
Purity	The active component mLIF has been shown to be >95% pure by SDS-PAGE. ESGRO® supplied 0.22 micron sterile filtered, and tested negative in mycoplasma tests.
Specific Activity	ESGRO® Supplement is assessed both on mouse embryonic stem cells (D3 & MBL-1) and on murine M1 myeloid leukemic cells(4). A standard of 50 Units is defined as the concentration of ESGRO® Supplement in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies(4). 10<sup>6</sup> units is equivalent to approximately 10 μg of pure protein, and is sufficient to treat 1L of ES cell culture media. <br /><br />Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 10<sup>8</sup> units/mg
Entrez Gene Number	NM_008501
Entrez Gene Summary	Leukaemia inhibitory factor is a cytokine that induces macrophage differentiation. Neurotransmitters and neuropeptides, well known for their role in the communication between neurons, are also capable of activating monocytes and macrophages and inducing chemotaxis in immune cells. LIF signals through different receptors and transcription factors. LIF in conjunction with BMP2 acts in synergy on primary fetal neural progenitor cells to induce astrocytes.
Gene Symbol	LIF Emfilermin CDF MLPLI HILDA D-FACTOR
UniProt Number	P09056
UniProt Summary	FUNCTION: SwissProt: P15018 # LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. SIZE: 202 amino acids; 22008 Da SUBCELLULAR LOCATION: Secreted. SIMILARITY: SwissProt: P15018 ## Belongs to the LIF/OSM family.
Media Form	Liquid
Stem Cell Type	Mouse Embryonic Stem Cells Induced Pluripotent Stem Cells

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes. The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid. For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.

Product Usage Statements

Usage Statement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY
Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.
The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.
For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.

Storage and Shipping Information
Storage Conditions	ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

Storage and Shipping Information

Storage Conditions

ESGRO® Supplement is shipped at ambient temperature. A cold pack may be included for customer relations, however it is truly not needed. Extensive stability tests have been performed with ESGRO® Supplement during development. This product is stable for at least 18 months from the date of manufacture, in the concentrated form or diluted in sterile tissue culture media, with no loss of activity on ES cells. For long term storage it is recommended that ESGRO® Supplement be stored at 4°C. Freeze-thawing will reduce potency. It is recommended that prior to use, ESGRO® Supplement should be diluted in sterile tissue culture media and aliquoted to a convenient concentration, then stored at 4°C. Freeze-thawing should be avoided. Product is stable for a minimum of 7 days at 37°C, 5% CO2 incubator during the culture of ES cells.

Packaging Information
Material Size	10? units
Material Package	1 mL

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