产品展厅
产品展厅
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| Description | |
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| Catalogue Number | FC010 |
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Chemicon® |
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| Description | Human Plasma Fibronectin Purified Protein |
| Overview | Human fibronectin (HFN) is suitable for use as an attachment factor in the propagation of cells in vitro when used to coat cell culture surfaces, including plastic ware, glassware, and microcarrier beads. |
| Product Information | |
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| Presentation | Liquid in 150 mM NaCl, 20 mM sodium phosphate, pH 7.3, sterile filtered, containing no preservatives. |
| Quality Level | MQ100 |
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| Application | This Human plasma fibronectin is a purified protein, used as an attachment factor suitable for cell propagation in vitro. |
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| Application Notes |
Immunochemical standard Cell attachment and proliferation assays on human endothelial cells, human keratinocytes and human dermal fibroblasts Suggested Procedure for Coating Cell Cultureware 1.Determine the amount of HFN needed to coat culture vessels by multiplying the total surface area (cm2) by the desired concentration (μg/mL) of HFN. Recommended amount is 2-10 μg/cm2. 2.Wet the surface of each culture vessel to be coated with a minimum amount of sterile balanced salt solution (serum and protein free) required to cover the entire area. 3.Introduce the proper CO2 atmosphere, if required. 4.Add the calculated amount of HFN to each culture vessel. 5.Allow HFN to adsorb to the surface of the vessel for 5-20 minutes. 6.Remove residual balanced salt solution before proceeding with standard cell culture procedures |
| Biological Information | |
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| Concentration | 1 mg/mL |
| Purity | Approximately 95%, as determined by SDS-PAGE. A double band of 220 kDa is present under reduced conditions. hFN is purified by affinity chromatography on gelatin agarose, followed by chromatography on heparin-agarose. Plasma from donors has been screened, and shown to be negative for HIV, HTLV, Hepatitis B and C. |
| Source | Human donor plasma. |
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| Entrez Gene Summary | This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. |
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| UniProt Summary |
FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity). SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1. SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix. TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age. PTM: Sulfated. SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains. |
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| Storage and Shipping Information | |
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| Storage Conditions | Maintain at 2-8°C for up to 6 months from date of receipt. Do not freeze. |
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| Material Size | 1 mg |
