HighYield T7 Digoxigenin RNA Labeling Kit-南京沃博生物科技有限公司

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HighYield T7 Digoxigenin RNA Labeling Kit

2023-02-16

产品详情

价格：4425
产品名称HighYield T7 Digoxigenin RNA Labeling Kit 查看相似产品 >

简介

产品信息

品牌	货号	名称	规格	单价
Jena Bioscience	RNT-101-DIGX	HighYield T7 Digoxigenin RNA Labeling Kit	30T x 20 μl	4425.00

产品简介

HighYield T7地高辛RNA标记试剂盒设计用于通过体外转录随机产生地高辛修饰的RNA探针。这种探针非常适合原位杂交和Northern Blot实验。标记原则类似于DIG RNA标记混合物（罗氏）和DIG RNA标签试剂盒（罗氏公司）的基本标记原则。使用优化的反应缓冲液和T7 RNA标记聚合酶混合物，DIG-11-UTP作为天然对应物UTP的替代物有效地并入RNA。35%DIG-11-UTP替代通常会在反应和标记效率之间实现最佳平衡。然而，使用单核苷酸格式可以轻松实现DIG-11-UTP/UTP比值的个别优化。由此产生的地高辛修饰RNA探针随后可通过HRP或AP修饰地高辛抗体检测。

该试剂盒含有足够的试剂，用于30个标记反应，每个反应20μl（35%DIG-11-UTP替代物，1 mM ATP，GTP，CTP，0.65 mM UTP，0.35 mM DIG-11-UTP）。

Content:
HighYield T7 RNA Labeling Polymerase Mix
2x 40 μl, incl. RNase inhibitor and 50 % glycerol (v/v)

HighYield T7 Reaction Buffer
1x 200 μl (10x), HEPES-based

ATP - Solution
1x 100 μl (100 mM)

GTP - Solution
1x 100 μl (100 mM)

CTP - Solution
1x 100 μl (100 mM)

UTP - Solution
1x 100 μl (100 mM)

DIG-11-UTP
1x 25 μl (10 mM)

T7 G-initiating control template (1.4 kbp)
1x 10 μl (200 ng/μl), 1.4 kbp PCR fragment plus T7 class III phi6.5 promotor resulting in approx. 1400 nt RNA transcript

PCR-grade water
1x 1.2 ml

DTT
1x 100 μl (100 mM)

To be provided by user
T7 Promotor-containing DNA template
RNA purification tools
RNAse-free DNAse I (optional)

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