产品信息

产品简介

     Plasmid-Safe™ ATP-Dependent DNase selectively hydrolyzes linear double-stranded (ds) DNA to deoxynucleotides at slightly alkaline pH and, with a lower efficiency, linear and closed-circular   single-stranded DNAs. The reaction is ATP-dependent, and does not affect   closed-circular supercoiled or nicked circular dsDNAs. The enzyme can be conveniently and completely heat-inactivated by a 30 minute incubation at   70°C. Plasmid-Safe DNase is useful as a final “cleanup” of DNA preparations  from plasmid and cosmid clones, to avoid the problems caused by contaminating  genomic DNA.
     粘粒、BAC、fosmid和质粒制备物中去除污染的细菌染色体DNA。该酶将从末端逐步降解线性DNA；闭环DNA(例如质粒)不具有游离末端，因此不会被降解。这些特性使得Plasmid-Safe ATP-Dependent DNase成为BAC和fosmid纯化方案的理想选择，例如用于鸟枪测序，以及其他需要高纯度DNA的应用。
优点：
- Digest contaminating linear DNA without digesting nicked or closed-circular dsDNA or     supercoiled DNA (plasmids, fosmids, etc.)；
- Use as a final purification step for plasmid, cosmid,  fosmid, and BAC vector and clone     preparations
- Utilize whenever protecting circular dsDNA is important
- 消化污染的线性DNA，而不消化有缺口或封闭的圆形dsDNA或超螺旋DNA（质粒，粘粒等）；
- 用作质粒，cosmid, fosmid，BAC载体和克隆制剂的最终纯化步骤；
- 在保护重要环状dsDNA时使用.
组成成分：
? Plasmid-Safe™  ATPDependent DNase （10U/μL）：1mL； -20°C
? Plasmid-Safe™ 10X   Buffer：5mL；-20°C
? ATP Solution (25 mM)：2m L； -20°C